Catalase Lab

Lab Report

Catalase Enzyme Lab

Catalase Enzyme Lab

Background:
Catalse is an enzyme normally found in more plants and animals. Its purpose is to defend polish off any toxic substances by destroying them. It is also use by some cells to destroy cellular waste. This lab will demonstrate the effect of temperature and pH on the action of this enzyme. The substrate of the enzyme will be hydrogen hydrogen peroxide (H2O2). Catalase works by the following mechanism.
2H2O2 ( 2H2O + O2
What is the effect of temperature and pH on the activity of catalase?
Aims:
Demonstrate and observe the effect of surface field of honor of the enzyme, temperature and pH on the activity of catalase
Materials:
1M. NaOHHot Plate10 ml graduated cylinder
1M. HClIcedistilled water
3% H2O22 full-size beakerspotato
6 test subway systemsForcepsliver
Test tobacco pipe rack3 micropipettesmortar and pestle
3 thermometerknifeStop watch
Test subway holderStirring rod

Part A Normal Catalase Reaction

guessing:

modest potato will have the highest rate of reaction

part:

1- Take two clean test subways and mark them 1 and 2.

2- Place a piece of potato in tube 1.

3- effuse 2ml of hydrogen peroxide to tube 2, and determine temperature.

4- Pour content of tube 2 to tube 1, and observe.

5- Pour off the liquid in tube 1, leaving the piece of potato.

6- contribute another 2 ml of hydrogen peroxide to tube 1, and observe.





7- Using the mortar and pestle, crush a piece of potato and place it in a clean test tube (3).

8- Pour 2ml of hydrogen peroxide into tube 3, and observe.

Data accrual:

Quantitative

Table 1: Recyclability and surface area effects on enzymatic activity

|Type of reaction |Uncrushed potato (control) (22 c) |Recycled potato (catalase) |Crushed Potato |
|Rate of reaction |3 |1...If you want to rile a full essay, order it on our website: Orderessay



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